Iwai North America Inc.
541 Taylor Way Suite# 4
San Carlos, CA 94070
Phone : (650) 486-1541
Fax : (650) 394-8638
Open weekdays 9 AM-6 PM (PST)
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TASCL™ Overview
TASCL™ (Tapered Stencil for Cluster Culture) microplates are an innovative 3D culture device developed to easily and efficiently create homogenous multicellular 3D spheroids or embryoid bodies (EB). Extensively tested with Induced pluripotent stem (iPS) cells, TASCL is expected to play a crucial role in regenerative medicine and in vitro drug screening that can provide patient specific and disease-specific precision medicine research.
iPS cells cultured using TASCL created multicellular spheroids or EB containing three germ layers. These EB biomimic the structure and function better than individual cells in 2D, making them suitable for studies in metabolism, toxicity, and regenerative medicine.
KEY Characteristics
• Mass produce 600-1000 homogenous spheroids per well in one seeding operation.
• Design of the well allows for nutrients and oxygen to circulate, making it ideal for long-term observation and differentiation studies.
• High density well design allows for less usage of medium and reagents.
TASCL Principles and Technology
TASCL is a 3D microfabricated microplate made of silicone rubber with surface treatment technology. Its patented design of dense array of pass-through holes and inclined walls allows for all seeded cells to settle into the holes. In addition, its thin membrane keeps the cells within these holes, but allows for nutrients and oxygen to circulate through.
TASCL microplates are provided on a pre-set culture insert and ready-to-use in a 6-well plate.
Procotol
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Proof of Concept: Multicellular 3D Spheroids - Embryoid Bodies (EB)
Size distribution of EB cultured in TASCL
References
1.Yukawa, H. et al. Embryonic body formation using the tapered soft stencil for cluster culture device. Biomaterials 32, 3729–3738 (2011).
2.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture. Cell Med 9, 35–44 (2017).
3.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device. Cell Med 8, 47–56 (2015).
4.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device. Cell Med 7, 67–74 (2015).
Cells Tested
•Human iPS cell lines.
Human iPS cell lines.•Cardiomyocyte derived from human iPS cells (healthy and diseased).
Cardiomyocyte derived from human iPS cells (healthy and diseased).•HepG2 (Hepatic)
HepG2 (Hepatic)•MIN6 (Pancreatic)
MIN6 (Pancreatic)•Human derived stem cells
Human derived stem cells•Human chondrocytes, knee
Human chondrocytes, knee•Mouse embryotic stem cell lines
Mouse embryotic stem cell lines•Mouse primary liver cell
Mouse primary liver cell•Mouse primary cardiomyocyte
Mouse primary cardiomyocyte