iMatrix-511 is an innovative cell culture matrix compatible with a wide variety of cell types, and exceptionally well suited for pluripotent stem cells. This product is comprised of recombinant Laminin-511 E8 protein fragments which permit ES/iPS cells to be maintained in xeno-free culture conditions, enable the passaging of single cells, and provide greater adhesion than full-length Laminin, Vitronectin or Matrigel¹.
With the conventional method, ES/iPS cells die when dispersed into single cells, making it necessary to maintain a cell colony when passaging.
Laminin-511 is a major component of the basement membrane used as a scaffold for pluripotent stem cells (ES/iPS cells), as it binds to integrin on cell surfaces. However Laminin-511 is a large protein (800kDa) composed of three chains (α-, β-, and γ-) forming a supramolecular aggregate, making it difficult to produce recombinantly. In order to overcome this challenge, Laminin-511 proteins were fragmented to find the smallest integrin-binding component and the E8 fragment was identified as the most promising result. In fact, hES cells were found to adhere more strongly to the E8 fragment than to the full-length protein.
In the study referenced above, the Laminin-511 E8 fragment and other cell culture substrates were compared for their strength in adhering to hES cells. The horizontal axis of the graph shows the concentration of cell culture substrate, and the vertical axis, the OD value (optical density at 570nm). This result shows that the Laminin-511 E8 fragment adheres to cells more strongly than its competitors.
In the same study cited above, Human ES cells were passaged on Laminin-511 E8 fragment and other cell culture substrates. Upon singly dispersing human ES cell colonies at the time of passage, it was confirmed that single cells adhere rapidly to Laminin-511 E8 and proliferate immediately.
Maintain and Culture Single ES/iPS Cells with Greater Propagation
Using the Laminin-511 E8 fragment as a cell culture matrix enables you to culture single ES/iPS cells, thereby reducing the time required to optimize culture methods and drastically enhancing efficiency of cell cultures.
Culture more ES/iPS cells using Laminin-511 E8 fragment
The graph above compares the numbers of ES/iPS cells cultured by the conventional method (colony) for 30 days with those cultured by Laminin-511 E8 fragment. The results confirm that the number of cells multiplied by at least 200 when Laminin-511 E8 fragment was used.
**For Research Use Only. Not For Use in Diagnostic Procedures**
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES
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