BIOMIMESYS® Oncology is a new generation of mimetic hydroscaffold for 3D cell culture. The physicochemical properties (composition and architecture) of the ECM play a critical role in regulating important cellular functions such as spreading, migration, proliferation, and differentiation. BIOMIMESYS® Oncology “Hydroscaffold” made of hyaluronic acid (HA) and type I collagen crosslinked with adipic acid dihydrazide (ADH), mimics the microenvironment of tumor cells thus creating the ideal condition for tumor cells to grow in 3D. Furthermore this scaffold provided cell binding sites with CD44 and RHAMM allowing cell-matrix and cell-cell interactions.

​Composition:

• Hyaluronic acid (HA) (1.6MDa) – Provides binding sites for CD44 and RHAMM cell surface receptors
• Adipic acid dihydrazide (ADH) as crosslinker
• Collagen Type I

Physicochemical Features:

• Young’s modulus: 1.1kPa – different stiffness is available by customization
• Porosity: 155±35µm
Key Features
  • Provides binding sites for CD44 & RHAMM cell surface receptors
  • Customizable
  • Easy to use (ready to use 96 / 384 sterile plate)
  • Compatible with all standard downstream analytical technologies
  • Co-culture (tumor cells - normal cells - immune cells)
  • Cancer cells proliferate and aggregate to form spheroid from the 5th day of culture
  • Long term culture (at least 28 days)
  • In vivo implantation possible (digestible hyaluronic acid) 
  • Ideal for high throughput screening (HTS) with 3D fluorescence imaging - Phenotypic Screening
References

1.Simon, T. et al. Direct effect of bevacizumab on glioblastoma cell lines in vitro. Neuromolecular Med 16, 752–771 (2014).

2.Demange, E. et al. Survival of cord blood haematopoietic stem cells in a hyaluronan hydrogel for ex vivo biomimicry. J Tissue Eng Regen Med 7, 901–910 (2013).

3.Coquerel, B. et al. Elastin-derived peptides: matrikines critical for glioblastoma cell aggressiveness in a 3-D system. Glia 57, 1716–1726 (2009).

4.David, L. et al. Collagens, stromal cell-derived factor-1alpha and basic fibroblast growth factor increase cancer cell invasiveness in a hyaluronan hydrogel. Cell Prolif 41, 348–364 (2008).

5.David, L. et al. Hyaluronan hydrogel: An appropriate three-dimensional model for evaluation of anticancer drug sensitivity. Acta Biomaterialia 4, 256–263 (2008).

6.David, L. et al. Reticulated hyaluronan hydrogels: a model for examining cancer cell invasion in 3D. Matrix Biology 23, 183–193 (2004).
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Iwai North America Inc.
541 Taylor Way Suite# 4
 San Carlos, CA 94070
 Phone : (650) 486-1541
Fax : (650) 394-8638
 Open weekdays 9 AM-6 PM (PST)
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BIOMIMESYS® is an organ-specific Extracellular Matrix (ECM) formed by the crosslinking reaction of hydrosoluble modified hyaluronic acid (HA) and other ECM components (collagens, fibronectin, etc.) with ADH (Adipic acid dihydrazide), to create a "Hydroscaffold" 3D cell culture system.
About BIOMIMESYS® Oncology
Ready to use

BIOMIMESYS® Liver is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.

Successfully Tested Cells

Cancer Cells Lines & Primary Tumors
Human brain metastasis: SA87
Human breast adenocarcinoma : MCF-7
Human breast carcinoma : CAL-51
Human cervix adenocarcinoma : HeLa
Human colorectal adenocarcinoma : DLD-1, HT29, Caco-2
Human glioblastoma : CB109 / CB74 / CB191
Human liver hepatocellular carcinoma : HepG2
Human liver hepatoma : PLC / PRF-5
Human lung carcinoma : NCI-H460
Human osteosarcoma : SaOs
Human ovarian carcinoma : IGROV-1
Human pancreas carcinoma : PANC-1
Human prostate cancer : PC3

* Proof of Concept
SPHEROID GROWTH, VIABILITY AND CELL PROLIFERATION
VISUALISATION OF EXTRACELLULAR MATRIX SECRETION IN CANCER CELLS
Compatible Technologies

BIOMIMESYS® hydroscaffold has many properties (transparent, porous & biodegradable) that make it ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix by a gentle and rapid procedure, meaning that cells cultured in BIOMIMESYS® may be analyzed using all technologies as shown below.
TRANSPARENT --- Microscopy, Plate Reader (OD, fluorescence, luminescence)
POROUS --- PCRs, Western-Blot, ELISA
BIODEGRADABLE --- Flow cytometry
SOLID --- Histology
Ready to use

BIOMIMESYS® Oncology is EASY & READY TO USE. Upon receiving the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well. To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto the surface of the matrix.

Pricing

BIOMIMESYS® Liver is sold for RESEARCH USE ONLY.
Request a Free Sample

Please contact us for more information about BIOMIMESYS Oncology. A representative from our sales team will be in contact with you as soon as possible.